Shiitake Mushroom Liquid Culture Syringe

Inoculating a Mushroom Growing Kit injection port grain bag with a liquid culture syringe is a common method for growing mushrooms. The following is a brief description of the process:

1. Start by sterilizing your work area, your hands, and all equipment you will be using. This will help prevent contamination.

2. Shake the liquid culture syringe to distribute the mycelium evenly throughout the liquid.

3. Locate the injection port on the grain bag. This is usually a small self-healing rubber port on the side of the bag.

4. Wipe the injection port with an alcohol swab to sterilize it.

5. Remove the cap from the syringe and insert the needle into the injection port at a slight angle. Be careful not to push the needle in too far, as this could damage the bag.

6. Slowly depress the plunger on the syringe to inject the liquid culture into the bag. Try to distribute the culture evenly throughout the grain.

7. Withdraw the needle and replace the cap on the syringe.

8. Repeat this process with any other bags you want to inoculate. Be sure to sterilize the needle in between inoculating bags.

9. Place the inoculated bags in a warm, dark place and wait for the mycelium to grow. If you have a still air box or flow hood, that would be optimal for minimizing contamination. Depending on the species of mushroom, colonization can take anywhere from a few days to a few weeks.

By following these steps, you should be able to successfully inoculate an injection port grain bag with a liquid culture syringe and begin growing your own mushrooms. For best results be sure your grain bag is fully colonized before transfer.

Mushroom liquid culture syringes are used in the process of growing mushrooms for commercial or personal use. Liquid cultures can be used to inoculate substrates for the growth of various types of mushrooms, and the syringes provide a convenient and sterile way to transport and dispense the liquid culture.

When storing mushroom liquid culture syringes, it is important to ensure that they are kept in a sterile and controlled environment to maintain their shelf life. The ideal storage temperature for mushroom liquid culture syringes is between 35-45°F (2-7°C) to prevent bacterial and fungal growth. It is important to avoid freezing or exposing the syringes to extreme heat, as this can damage the spores and reduce their viability.

In addition to temperature control, it is important to protect the syringes from light exposure. Light can damage the spores, and exposure to direct sunlight or fluorescent light can cause a decrease in spore viability. As such, it is recommended to store the syringes in a dark place, such as a refrigerator or dark cabinet.

The shelf life of mushroom liquid culture syringes can vary depending on the specific strain of mushroom, as well as the conditions in which they are stored. On average, most mushroom liquid culture syringes have a shelf life of 6-12 months when stored properly. It is important to check the expiration date of the syringe before use and to discard any syringes that have passed their expiration date.

All of our liquid culture syringes are sterilized and filled in a lab environment. We take great precautions to ensure your products are viable upon arrival. However, some contamination can occur with shipping damage.

When you receive any of our products, it’s best practice to set them aside for 4 to 7 days before using. Inspect the product regularly for signs of contamination and if you notice something, we’ve got you covered! 

All of our products come with our sterile guarantee. If something gets lost or damaged during shipping, we’ll replace it at our expense. Just contact us.