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Mushroom Monotub Kit


Mushroom Monotub Kit includes the following:

  • Monotub and lid formatted for filtration
  • Choice of 1 – 3lb. Bag of Sterilized Grain (Rye Berries, Millet or Combination)
  • 3 – 5lb. Bags of Substrate for Dung Loving Mushrooms
  • 1 – 5lb. Bag of Coco Coir
  • Spray Bottle
  • Filter Tape
  • 12 Filters
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SKU TUB-01-COM Categories ,
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Need help choosing the right type of grain for spawn?

Read our article about choosing the best grain for mushroom spawn.

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Inoculating a Mushroom Growing Kit injection port grain bag with a liquid culture syringe is a common method for growing mushrooms. The following is a brief description of the process:

  1. Start by sterilizing your work area, your hands, and all equipment you will be using. This will help prevent contamination.

  2. Shake the liquid culture syringe to distribute the mycelium evenly throughout the liquid.

  3. Locate the injection port on the grain bag. This is usually a small self-healing rubber port on the side of the bag.

  4. Wipe the injection port with an alcohol swab to sterilize it.

  5. Remove the cap from the syringe and insert the needle into the injection port at a slight angle. Be careful not to push the needle in too far, as this could damage the bag.

  6. Slowly depress the plunger on the syringe to inject the liquid culture into the bag. Try to distribute the culture evenly throughout the grain.

  7. Withdraw the needle and replace the cap on the syringe.

  8. Repeat this process with any other bags you want to inoculate. Be sure to sterilize the needle in between inoculating bags.

  9. Place the inoculated bags in a cool, dark place and wait for the mycelium to grow. If you have a still air box or flow hood, that would be optimal for minimizing contamination. Depending on the species of mushroom, colonization can take anywhere from a few days to a few weeks.

By following these steps, you should be able to successfully inoculate an injection port grain bag with a liquid culture syringe and begin growing your own mushrooms. For best results be sure your grain bag is fully colonized before transfer.

To cultivate a successful mycelium growth, it is crucial to provide an ideal environment for your bag. Find a cool and shaded area where the temperature ranges between 55 to 75 degrees Fahrenheit (depending on strain). Give the bag a few weeks to colonize and observe the emergence of mycelium on the grain.

As the mycelium grows from the injection point, it will gradually spread outwards, forming a white layer on the grain. Once the mycelium has extended three to four inches in length, it is time to carefully break up the colonized grain and blend it with the uncolonized grain. This will facilitate the colonization process and quicken its growth.

The colonization process can take a few weeks to a month or more, depending on the species and storage conditions. Keep an eye on the bag and watch for the complete white layer of mycelium to form, indicating that your spawn is ready to use. With patience and a good environment, you can produce a healthy and robust mycelium culture.

Once the grain bags are fully colonized, break up the mycelium and grain by massaging the bags. Open the bags and layer the inoculated grain and moistened subsrate in the monotub. Start with a layer of substrate, followed by a layer of moist coco coir, and continue alternating until all materials are used up. The top layer should be coco coir.

Place the lid on the monotub, ensuring the holes and filters are properly aligned. The filters will allow for proper air exchange, which is crucial for mushroom growth.

Move the monotub to a location with indirect sunlight or ambient room light, and maintain a temperature of 65-75°F (18-24°C) and a relative humidity of 80-95%. You can achieve the proper humidity by misting the inside of the tub with water and fanning for air exchange 2-3 times per day.

Within 7-14 days, you should start to see small mushroom pins forming. Continue misting and fanning as needed. Once the mushrooms have matured (usually when the veil under the cap begins to break), gently twist and pull them from the substrate to harvest.

After the first flush of mushrooms, you may get additional flushes. Allow the substrate to rest for a week, continue misting and fanning as before. You can expect 2-4 flushes of mushrooms before the substrate is exhausted.

Remember that growing mushrooms can be an art as much as a science. You may need to make adjustments to your process based on your specific environment and conditions. Don’t be discouraged if your first attempt isn’t perfect; practice and experimentation will lead to better results.

All of our injection port grain bags are sterilized and filled in a lab environment. We take great precautions to ensure your products are viable upon arrival. However, some contamination can occur with shipping damage.

When you receive any of our products, it’s best practice to set them aside for 4 to 7 days before using. Inspect the product regularly for signs of contamination and if you notice something, we’ve got you covered! 

All of our products come with our sterile guarantee. If something gets lost or damaged during shipping, we’ll replace it at our expense. Just contact us.

Introducing the Mushroom Monotub Growing Kit – your one-stop solution for an effortless and rewarding mushroom cultivation experience! This all-inclusive kit is designed to provide you with everything you need to grow a thriving, bountiful harvest of dung loving mushrooms in the comfort of your own home.

Our Mushroom Monotub Growing Kit includes:

  1. Monotub with Air Filtration and Lid: Say goodbye to contamination risks and hello to a clean, healthy growing environment! Our monotub is equipped with air filtration ports, ensuring your mushrooms get the fresh air they need while keeping unwanted contaminants at bay. The secure, easy-to-use lid maintains optimal humidity levels, creating the perfect environment for your fungi to flourish.
  2. 1 3lb. Bag of Grain: Packed with essential nutrients, our rye and millet grains provides a superior substrate base for your mushrooms. The mixture promotes strong mycelial growth and improves the overall yield of your mushroom harvest. Comes in an injection port bag for easy colonization.
  3. 3 5lb. Bags of Substrate: Our expertly crafted substrate mix contains organic manure, gypsum, vermiculite, and coco coir, providing a nutrient-rich and well-draining environment for your mushrooms to thrive. This carefully formulated blend ensures strong, healthy growth and an abundant harvest.
  4. 1 5lb. Bag of Coco Coir: The addition of high-quality coco coir helps maintain proper moisture levels in your monotub, promoting ideal growing conditions for your mushrooms. This versatile and eco-friendly ingredient also aids in maintaining a robust and healthy mycelial network.

Whether you’re an experienced cultivator or just starting your mushroom-growing journey, the Mushroom Monotub Growing Kit is the perfect choice for a hassle-free, successful harvest. With our comprehensive kit and easy-to-follow instructions, you’ll be well on your way to enjoying the satisfaction of homegrown mushrooms in no time! Order your Mushroom Monotub Growing Kit today and embrace the magic of mushroom cultivation.

Additional information

Weight 395 oz
Dimensions 22 × 13 × 17 in

Rye Berries, Millet, Rye & Millet Combo


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